BIOSIS / 2003:317841

AN

    2003:317841  BIOSIS

DN

    PREV200300317841

TI

    Downregulation of islet hormone-sensitive lipase during long-term
    high-fat feeding. 

AU

    Winzell, Maria Sorhede [Reprint Author]; Holm, Cecilia; Ahren, Bo

CS

    Department of Cell and Molecular Biology, Sec. for Molecular Signalling,
    Biomedical Center, Lund University, S-221 84, Lund, Sweden
    Maria.Sorhede_Winzell@medkem.lu.se

SO

    Biochemical and Biophysical Research Communications, (May 2 2003) Vol.
    304, No. 2, pp. 273-278. print. 
    CODEN: BBRCA9. ISSN: 0006-291X. 

DT

    Article

LA

    English

ED

    Entered STN: 9 Jul 2003
    Last Updated on STN: 9 Jul 2003

AB

    Lipid accumulation in pancreatic beta-cells during high-fat (HF) feeding
    may be involved in inducing a defective insulin secretion due to
    lipotoxicity.  Hormone-sensitive lipase (HSL) is expressed and
    active in beta-cells, but its importance for islet dysfunction during the
    development of type 2 diabetes is not known.  In this study, prolonged
    HF feeding of C57BL/6J mice, resulted in decreased HSL expression in
    islets, representing only 25 +- 4% of the levels observed in controls.
    This was paralleled by triglyceride accumulation and blunted insulin
    secretion both in vivo and in vitro.  After switching the HF diet to a LF
    diet, HSL expression increased 10-fold compared to the HF fed mice.
    This was accompanied by reduced triglyceride levels and a restored insulin
    secretion.  These results support the notion that HSL plays a critical
    role in the regulation of intracellular triglyceride levels in beta-cells,
    and that down-regulation of the enzyme may serve to protect against fatty
    acid-induced islet dysfunction.

CC

    Cytology - Animal   02506
    Biochemistry studies - Proteins, peptides and amino acids   10064
    Biochemistry studies - Lipids   10066
    Enzymes - General and comparative studies: coenzymes   10802
    Metabolism - Metabolic disorders   13020
    Nutrition - General studies, nutritional status and methods   13202
    Endocrine - General   17002
    Endocrine - Pancreas   17008
    Toxicology - General and methods   22501

IT

    Major Concepts
       Endocrine System (Chemical Coordination and Homeostasis); Enzymology
       (Biochemistry and Molecular Biophysics); Nutrition

IT

    Parts, Structures, & Systems of Organisms
       pancreatic beta-cell: endocrine system

IT

    Diseases
       type 2 diabetes: endocrine disease/pancreas, metabolic disease
       Diabetes Mellitus, Non-Insulin-Dependent (MeSH)

IT

    Chemicals & Biochemicals
       fat: nutrient; fatty acid; hormone-sensitive lipase:
       downregulation; insulin; lipid: toxicity; triglyceride

ORGN

    Classifier
       Muridae   86375
    Super Taxa
       Rodentia; Mammalia; Vertebrata; Chordata; Animalia
    Organism Name
       mouse (common)
    Taxa Notes
       Animals, Chordates, Mammals, Nonhuman Vertebrates, Nonhuman Mammals,
       Rodents, Vertebrates

RN

    9001-62-1 (hormone-sensitive lipase)
    9004-10-8 (insulin)